The Active Peptide from Shark Liver (APSL) was expressed in E.coli BL21 cells.The cDNA encoding APSL protein was obtained from shark regenerated Garters hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector.The expressed fusion protein was purified by Ni-IDA affinity chromatography.SDS-PAGE and HPLC analysis showed the purit
Bruchus pisorum is an insect pest causing major damage to pea seeds worldwide.Control is difficult and limited resistance is available.In this work we studied the effects Personal Electronics of pollen and pod source on insect fecundity and oviposition by comparing resistant and susceptible Pisum spp.accessions and non-host (Lathyrus sativus and Vi
Abstract China, the European Union, and the United States are the three largest digital economies in the world.This article compares the antitrust regulation of the digital economy in the three regions after the 2000s.It argues that over time, Hoodie the antitrust regulation of the digital economy in the three regions tends to converge along three
Specular reflection algorithm (SRA) was a single population meta-heuristic algorithm inspired by the physical function of mirror.However, similar to most of meta-heuristic algorithms, it had the disadvantages of weak population diversity, stagnation in local optimal and low convergence rate.In order to overcome these shortcomings, a chaotic multi-s